mas complementation plasmid original reference Search Results


human ace2  (Addgene inc)
96
Addgene inc human ace2
A . Schematic representation of the BiFC assay. The c-Jun and c-Fos proteins were fused to the Nt and Ct ends of the VFP, respectively, to create the c-Jun/VN and c-Fos/VC chimeras (Jun and Fos). Reconstitution of the structure of the VFP (represented in green), and thus its fluorescence properties, occurs exclusively after c-Jun and c-Fos interaction. B . Schematic representation of the BiMuC assay. To study SARS-CoV-2 membrane fusion properties. Hek-293T cells were transfected either with SARS-CoV-2 S and Jun or with <t>ACE2</t> and Fos. Next, these two cell populations were combined. Successful interaction between SARS-CoV-2 S and ACE2 leads to the fusion of the cellular membranes, the creation of a syncytium, and the reconstitution of the VFP native structure (depicted in green). On the contrary, inhibition of any of the cellular or viral processes that lead to membrane fusion (from synthesis and maturation of the S protein to its interaction with ACE2) will result in the absence of a fluorescent signal. C . For the cell-cell fusion assay, Hek-293T cells with the indicated plasmid combinations. The next day cells were counted, mixed, and seeded into 96 well plates. After 48 hrs, fluorescence was measured. Bars show the average and standard deviation of at least three independent experiments. Green bars denote those samples with fluorescence levels comparable to the positive control (Jun-Fos). D . To facilitate visualization of the TMPRSS2 effect on viral fusion RFUs were plotted as the percentage over the Jun-S Fos-ACE2 sample.
Human Ace2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mas+complementation+plasmid+original+reference/bio_rxiv__2022__11__08__515589-84-10-19?v=Addgene+inc
Average 96 stars, based on 1 article reviews
human ace2 - by Bioz Stars, 2026-08
96/100 stars
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mas complementation plasmid original reference  (Addgene inc)
93
Addgene inc mas complementation plasmid original reference
A . Schematic representation of the BiFC assay. The c-Jun and c-Fos proteins were fused to the Nt and Ct ends of the VFP, respectively, to create the c-Jun/VN and c-Fos/VC chimeras (Jun and Fos). Reconstitution of the structure of the VFP (represented in green), and thus its fluorescence properties, occurs exclusively after c-Jun and c-Fos interaction. B . Schematic representation of the BiMuC assay. To study SARS-CoV-2 membrane fusion properties. Hek-293T cells were transfected either with SARS-CoV-2 S and Jun or with <t>ACE2</t> and Fos. Next, these two cell populations were combined. Successful interaction between SARS-CoV-2 S and ACE2 leads to the fusion of the cellular membranes, the creation of a syncytium, and the reconstitution of the VFP native structure (depicted in green). On the contrary, inhibition of any of the cellular or viral processes that lead to membrane fusion (from synthesis and maturation of the S protein to its interaction with ACE2) will result in the absence of a fluorescent signal. C . For the cell-cell fusion assay, Hek-293T cells with the indicated plasmid combinations. The next day cells were counted, mixed, and seeded into 96 well plates. After 48 hrs, fluorescence was measured. Bars show the average and standard deviation of at least three independent experiments. Green bars denote those samples with fluorescence levels comparable to the positive control (Jun-Fos). D . To facilitate visualization of the TMPRSS2 effect on viral fusion RFUs were plotted as the percentage over the Jun-S Fos-ACE2 sample.
Mas Complementation Plasmid Original Reference, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mas+complementation+plasmid+original+reference/pmc11237470__msphere__00005___24___s0002-59-9-28?v=Addgene+inc
Average 93 stars, based on 1 article reviews
mas complementation plasmid original reference - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier

Image Search Results


A . Schematic representation of the BiFC assay. The c-Jun and c-Fos proteins were fused to the Nt and Ct ends of the VFP, respectively, to create the c-Jun/VN and c-Fos/VC chimeras (Jun and Fos). Reconstitution of the structure of the VFP (represented in green), and thus its fluorescence properties, occurs exclusively after c-Jun and c-Fos interaction. B . Schematic representation of the BiMuC assay. To study SARS-CoV-2 membrane fusion properties. Hek-293T cells were transfected either with SARS-CoV-2 S and Jun or with ACE2 and Fos. Next, these two cell populations were combined. Successful interaction between SARS-CoV-2 S and ACE2 leads to the fusion of the cellular membranes, the creation of a syncytium, and the reconstitution of the VFP native structure (depicted in green). On the contrary, inhibition of any of the cellular or viral processes that lead to membrane fusion (from synthesis and maturation of the S protein to its interaction with ACE2) will result in the absence of a fluorescent signal. C . For the cell-cell fusion assay, Hek-293T cells with the indicated plasmid combinations. The next day cells were counted, mixed, and seeded into 96 well plates. After 48 hrs, fluorescence was measured. Bars show the average and standard deviation of at least three independent experiments. Green bars denote those samples with fluorescence levels comparable to the positive control (Jun-Fos). D . To facilitate visualization of the TMPRSS2 effect on viral fusion RFUs were plotted as the percentage over the Jun-S Fos-ACE2 sample.

Journal: bioRxiv

Article Title: Identification of small molecules with virus growth enhancement properties

doi: 10.1101/2022.11.08.515589

Figure Lengend Snippet: A . Schematic representation of the BiFC assay. The c-Jun and c-Fos proteins were fused to the Nt and Ct ends of the VFP, respectively, to create the c-Jun/VN and c-Fos/VC chimeras (Jun and Fos). Reconstitution of the structure of the VFP (represented in green), and thus its fluorescence properties, occurs exclusively after c-Jun and c-Fos interaction. B . Schematic representation of the BiMuC assay. To study SARS-CoV-2 membrane fusion properties. Hek-293T cells were transfected either with SARS-CoV-2 S and Jun or with ACE2 and Fos. Next, these two cell populations were combined. Successful interaction between SARS-CoV-2 S and ACE2 leads to the fusion of the cellular membranes, the creation of a syncytium, and the reconstitution of the VFP native structure (depicted in green). On the contrary, inhibition of any of the cellular or viral processes that lead to membrane fusion (from synthesis and maturation of the S protein to its interaction with ACE2) will result in the absence of a fluorescent signal. C . For the cell-cell fusion assay, Hek-293T cells with the indicated plasmid combinations. The next day cells were counted, mixed, and seeded into 96 well plates. After 48 hrs, fluorescence was measured. Bars show the average and standard deviation of at least three independent experiments. Green bars denote those samples with fluorescence levels comparable to the positive control (Jun-Fos). D . To facilitate visualization of the TMPRSS2 effect on viral fusion RFUs were plotted as the percentage over the Jun-S Fos-ACE2 sample.

Article Snippet: The Jun-Nt VFP (Jun) and Fos-Ct VFP (Fos) and the human ACE2 and TMPRSS2 expressing plasmids were obtained from Addgene (#22012, #22013, 1786, and #53887 respectively).

Techniques: Bimolecular Fluorescence Complementation Assay, Fluorescence, Membrane, Transfection, Inhibition, Cell-Cell Fusion Assay, Plasmid Preparation, Standard Deviation, Positive Control